Application

One Component Chemiluminescent Membrane / Blotting Peroxidase Substrate is luminol-based and can detect horseradish peroxidase at high sensitivity levels (low picogram to femtogram). It provides superior sensitivity and convenience compared to competitor products. The substrate is supplied as one component. It requires no mixing and is ready to use, thus eliminating mixing errors. CPSOC substrate may be used for immunoblots, western blots, dot blots and any blotting application utilizing horseradish peroxidase (HRP)-conjugates. The substrate can be used with various blocking buffers and on nitrocellulose or PVDF membranes. Such blots will exhibit low backgrounds. Detection and analysis may be done by CCD imaging systems or x-ray film.